The GlobalTown’s EZ-NeWMyo™ are high-performance micro vessel myograph systems for pharmacology and pathology studies of micro or small tubular biological tissues that affect vast physiological functions such as hypertension, diabetes, respiratory, GI, urinary or colonic diseases, etc. They are based on the GlobalTown’s proven, proprietary methodologies, EZ-Needle Myograph and EZ-Wire Myograph. Micro tubular tissues as small as 60 microns, such as mouse heart coronary and messenteric rings, have been efficiently investigated, with sensitivity up to 0.2-0.5mg, using buffer as little as 0.5ml, which is unprecedented in the field and translates into drastic reduction (3-20x less) in vascular reagents and drugs needed for experiments, compared to Traditional Wire Myograph (TWM). The EZ-NeWMyo systems also feature proprietary micro vessel threading methodology, sensitive and robust isometric transducers (i.e., the –SW series with both high sensitivity and wide dynamic range), combined bio-sensing, fluorescence probing and myograph, versatility for different sized vessels, consistency in vessel tension and positions across channels and experiments, and efficient system setting up, operating, data collection and analyses, etc. They have outperformed TWM systems in almost all areas of vessel myograph (details available for potential customers only, please email us at info@globaltowns.com), but they are much more affordable than the TWM systems in the market.

New features include (1). embedded bio-sensors (NO, glucose and epinephrine) and fluorescence probe, for bio-sensing and fluorescence detection in micro vessel myograph; (2). automated (optional) or manual (one-push valve) buffer filling, emptying or washing.

• Pharmacolology & pathology studies of vascular rings as small as 60 microns, based on proprietary EZ-Needle and/or EZ-Wire Myograph with sensitivity up to 0.2-0.5mg in buffer as small as 0.5ml:
  - vascular smooth muscle functions
  - vascular endothelium cells functions
  - normalization: length-tension relationship
  - vessel reactivity & morphology
  - vessel pharmacology & pathology reactivity

• Suitable are the following micro, small or large tubular tissues from mice, rats, guinea pig, etc:
  - (mouse) coronary or messenteric arteries or veins from 60um ID up
  - renal arcuate arteries, lymph vessels, intestine or colon rings
  - bronchi and respiratory tract (lung & tracheal) tissues
  - ureter, urinary tract (bladder and corpus carernosum)

PARTIAL LIST OF RESEARCH INSTITUTIONS with EZ-NeWMyo or EZ-Bath SYSTEMS:

Bond University (Australia)
Georgia State University
Grand Valley State University
Linkoping University (Sweden)
Lithuania Kuanas Medical University (Lithuania)
University of Michigan
University of Missouri at Columbia
University of Missouri at Kansas City
University of New South Wales (Australia)
Neurogen, Inc.
University of North Texas
Ohio Northern University
University of Sunshine Coast (Australia)
University of Toronto (Canada)
University of Western Ontario (Canada)
...

The EZ-NeWMyo Systems Include the Following:

• Drug/Biosensor Manifold;
• Micro Vessel measure/Control Assembly (patent pending): MV-MC25 (standard) & MV-MC25SF (w/ bio-sensing & fluorescence option);
• Proprietary Micro Vessel Threaders: MVETH 100 (no microscope needed) and TWM-compatible MVETH 50 (need microscope);
• Micro Vessel Holders: MVW-60, -100, -200 or MVN-200, -400, -1000;
• Highly sensitive and robust i-FOT 50SW transducer w/ 0.5mg sensitivity and 0-100g range;
• Buffer pre-oxygenation and aeration reservoir and buffer distribution manifolds with integrated valves and automated options;
• Oxygenation/Aeration Manifold with easy controls, and Waste Manifold;
• Versatile, easy-to-use i-DAQ Data Acquisition System and Software i-DAQChat with data fitting functions;
• Option of bio-sensors and fluorescence probe;
• And necessary accessories;

The Two Modes of Operations, EZ-Needle and EZ-Wire Myograph, Are Easily Exchangeable:

Proprietary EZ-Needle Myograph: The fast micro vessel mounting (no microscope needed), uniform isometric preset tension throughout vessel, and 0.5ml minimal buffer and huge saving in drug costs (compared to all other vertical or horizontal vessel Wire Myograph systems) for vessels 200um and up, in 2ml baths.

Proprietary EZ-Wire Myograph: unique, proprietary method of micro-vessel wire threading, with proprietary MVETH-100 and MVETH-50 Threaders, and ultra smooth mounting and positioning, sensitive contractile signal relay, much less system and drug costs needed than Traditional Wire Myograph, for vessels 60um and up, in 2ml baths.

EZ-NeWMyo Advantages:Compared to Traditional Wire Myograph, the proprietary methodology and systems outperform in

                                     at least 7 areas, as summarized below:

• Quick, less-intrusive mounting of micro vascular rings, which translates into efficient and productive experiments;
• Consistent, centered point of tension signal transduction and vessel position with 2D+1 micro measurements and adjustments;
• High and robust tension sensitivity and dynamic range, with versatile data recording and analysis software;
• Fully modular design including exchangeable micro vessel holders;
• The optional of simultaneous myograph and bio-sensing and fluorescence probing;
• Unprecedented reduction (3-20x less) in amount of vascular reagents/drugs needed;
• Much less system acquisition costs (40-60% less), compared to Traditional Wire Myograph systems.

For details on these feature comparisons, please contact info@globaltowns.com or sales@globaltowns.com .

1.     Micro Tension/Force Transducers (included)

The i-FOT 10SW and i-FOT 50SW are specifically designed to have both high sensitivity (0.5mg) and wide dynamic range (0-100g), and well suited for the EZ-NeWMyo system. They are compatible with our BRAM-4B, i-BRIDGE transducers amplifiers, or BRAMDA-400/800 and i-DAQ 4/8 data acquisition systems. They can also be easily adapted to ADI and other suppliers’ systems. Other transducers in i-FOT Series with different range and sensitivity are also available.

2.     Bio-sensing of Nitric Oxide and Fluorescence Detection of Calcium and Membrane Potential (optional):

The EZ-NeWMyo systems provide capabilities for both bio-sensing and fluorescence measurement for blood vessel tissues. Our miniature biosensors and fluorescence probe are used seamlessly for simultaneously measuring nitric oxide (NO), epinephrine, norepinephrine, calcium and/or membrane potential of vessel rings under tension or drug perfusion. This allows studies of interactions between vascular muscle tone and biochemical release or perfusion, e.g. vascular endothelium NO and calcium-mediated vessel relaxation; or epinephrine- or adenosine-activated vascular regulation.

The bio-sensors and fluorescence probe are embedded as part of the micro vessel holding assembly, providing consistency and convenience in the experimental protocols without the need for external positioner. They are installed on sensor- and fluorescence-enabled Micro Vessel Measure & Control Assembly Bracket MV-MC25SF that also allows additional positioning of the bio-sensor or fluorescence probe. To include bio-sensor in your system order, please add “–SF” at the end of the system part number, and then specify what model of biosensor and probe are needed.